| 摘要 |
The microdialysis technique and device of the present invention can be used to study metabolic aspects of human and animal organs. When a microdialysis catheter is for example placed into the substance of a beating heart, there is always concern for problems with the catheter position. Further, there is always a risk for damage to heart tissue as well as to the catheter. The present invention is designed to avoid such damages, and a study has confirmed that data obtained from the epicardial probe of the present invention reflects the state of the myocardial metabolism. In anesthetized normoventilated pigs (n=20), a sternotomy was performed and a Gore-tex suture snare was placed around a branch of the left anterior descending (LAD) artery. One microdialysis probe was inserted in the myocardial tissue supplied by the snared LAD branch. Another microdialysis probe was placed on the epicardial surface above the myocardial probe. The protocol included four, ten minute ischemic periods (intervention group) followed by a forty minute ischemic period. The control group was only exposed to a forty minute ischemic period. It was possible to place the microdialysis probes of the present invention on the epicardial surface, and to recover samples reflecting the myocardial metabolic state of the heart. It was also possible to measure rapid changes in concentrations especially for lactate, where ischemia periods as short as ten minutes resulted in detectable increases. A similar pattern was found when analysing glucose and glycerol concentrations in paired samples. Data obtained from the device and method of the present invention, placed on the epicardial surface showed a similar pattern compared to data from a standard probe placed in the myocardium. Thus, the epicedial application of the present invention is useful for perioperative monitoring during cardiac surgery.
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