发明名称 |
VERFAHREN ZUR SELEKTION EINES EIN FREMDGEN ÜBEREXPRIMIERENDEN ESCHERICHIA COLI-STAMMS, SO SELEKTIONIERTE ESCHERICHIA COLI-MUTANTE UND VERFAHREN ZUR HERSTELLUNG EINES ENZYMS UND EINER VERBINDUNG UNTER VERWENDUNG DAVON |
摘要 |
The invention relates to a selection method of an Escherichia coli mutant strain which expresses an exogenous gene at a high level, wherein stress resistance such as hydrogen peroxide decomposition activity is used as an index, the Escherichia coli mutant strain selected thereby and production methods of enzyme using the strain and of useful compounds like amino acids (especially L-amino acid) and the like using the strain. <??>According to the selection method of the invention, an Escherichia coli mutant strain where gene expression amount does not decrease with passage can be obtained, and a compound using a plant-derived ammonia lyase can be efficiently produced. |
申请公布号 |
DE60332487(D1) |
申请公布日期 |
2010.06.17 |
申请号 |
DE2003632487 |
申请日期 |
2003.12.11 |
申请人 |
SHOWA DENKO K.K. |
发明人 |
YAZAKI, KAZUFUMI;AOKI, HIROBUMI;KAMACHI, HARUMI |
分类号 |
C12N1/21;C12N9/88;C12N15/60;C12P13/04 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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