L-SUCCINYL AMINOACYLASE AND PROCESS FOR PRODUCING L-AMINO ACID USING SAME

摘要

Disclosed is an L-succinyl aminoacylase comprising (a) a protein coded by a gene consisting of the base sequence disclosed in sequence no. 1; (b) a protein consisting of the amino acid sequence disclosed in sequence no. 2; (c) a protein coded by a polynucleotide hybridized with a base sequence complementary to the base sequence disclosed in sequence no. 1 under stringent conditions, and which possesses L-succinyl aminoacylase activity; or (d) an amino acid sequence, in which one or multiple amino acids are substituted, deleted, inserted, and/or added to a protein consisting of the amino acid sequence disclosed in sequence no. 2, and which possesses L-succinyl aminoacylase activity. This enzyme can efficiently produce a sterically bulky non-natural amino acid, such as L-tertiary leucine that is useful as a pharmaceutical intermediate.