发明名称 |
SiRNA SEQUENCE-INDEPENDENT MODIFICATION FORMATS FOR REDUCING OFF-TARGET PHENOTYPIC EFFECTS IN RNAi, AND STABILIZED FORMS THEREOF |
摘要 |
Modification formats having modified nucleotides are provided for chemically synthesised RNA, in particular duplex RNA. RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted RNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays. |
申请公布号 |
EP2195428(A2) |
申请公布日期 |
2010.06.16 |
申请号 |
EP20080831316 |
申请日期 |
2008.09.17 |
申请人 |
APPLIED BIOSYSTEMS, LLC |
发明人 |
PURI, NITIN;CHARLES, IRUDAYA;MAGDALENO, SUSAN;VLASSOV, ALEXANDER;BURNETT, CHRIS |
分类号 |
C12N15/11;C12N15/113 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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