发明名称 |
POLYMERASE DERIVED FROM THERMOCOCCUS MARINUS AND ITS USE |
摘要 |
PURPOSE: A DNA polymerase derived from Thermococcus marinus is provided to use in nucleic acid polymerization such as PCR. CONSTITUTION: A heat resistant Tma DNA polymerase isolated from Thermococcus marinus has an amion acid sequence of sequence number 12. The Tma DNA polymerase is optimally active at pH 7.0, 75°C, 0-20 mM of KCl, 0-15 mM of (NH_4)_2SO_4, and 12-16 mM of Mg^2+. A nucleic acid encoding the Tma DNA polymerase has nucleic acid sequence of sequence number 11. A method for manufacturing Tma DNA polymerase comprises: a step of preparing a vector expressing Tma DNA polymerase; a step of transforming the vector to microorganism; a step of culturing the transformant; and a step of collecting the proteins from the transformant.
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申请公布号 |
KR20100064731(A) |
申请公布日期 |
2010.06.15 |
申请号 |
KR20080123299 |
申请日期 |
2008.12.05 |
申请人 |
SUNGKYUNKWAN UNIVERSITY FOUNDATION FOR CORPORATE COLLABORATION |
发明人 |
KWON, SUK TAE;BAE, HEE JIN |
分类号 |
C12N15/52;C12N1/20;C12N9/12 |
主分类号 |
C12N15/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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