COMBINATIONAL THERAPEUTICS FOR TREATMENT OF PROSTATE CANCER USING EPOXY ENCAPSULATED MAGNETIC PARTICLES AND RNAI MEDICINE

摘要

The present invention provides compositions and methods for treatment of prostate cancer using siRNA cocktail sequences targeting multiple disease causing genes, and a combined regimen with both RNAi therapeutics and EEMP encapsulated magnetic nanoparticle therapeutics. The invention includes: 1) The algorithm for designing siRNA drug API (active pharmaceutical ingredient); 2)The compositions of multiple siRNA inhibiting expression of the multiple cancer causing genes; 3) The methods of designing siRNA duplexes with different lengths, different ends and to target the same genes from both human and mouse cells; 4) The methods of formulating various siRNA cocktail which are able to inhibit tumorigenic and angiogenesis; 5) The methods of using histidine-lysine copolymer (HKP), PAMAM dendrimer and Liposomal (DOTAP) carrier to enhance siRNA delivery into the cutaneous tissue; and 6) The methods of using two disease relevant mouse xenograft models to validate the siRNA cocktail for its efficacy on controlling tumor growth.