| 摘要 |
The invention discloses the microspheres comprising the following structure in the composition thereof and the uses as tracers and fluorescent standard calibration products. wherein, groups A, B and C are linked to hydrocarbon chain , and at least one of A, B and C has the structure of—N═R—, in which R is a residue after the reaction of an aldehyde-type cross-linking substance, and the others of A, B and C are—NH2 structure. The microspheres comprising the above structure have an obvious phenomenon of self-fluorescence, and the fluorescence output of each microsphere is significantly larger than the fluorescence outputted by the microsphere embedded with fluorescent substances. The microspheres have toleration to light-bleach and the dependency on other environments without modifying the surface property of the microspheres themselves. The invention further discloses the preparation method of the above fluorescent microspheres. The fluorescence color of the fluorescent microspheres can be adjusted by selecting different aldehyde-type substances or the combinations thereof, and the fluorescence intensity of the microspheres can be controlled not only by the addition of the aldehyde-type substances and the time of the cross-linking reaction, but also by adjusting the size of the particle diameters of the microspheres.
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