| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for discriminating the edwardsiellasis of flatfish, enabling flatfish to be efficiently bred through discriminating flatfish tending to suffer from edwardsiellasis using a specific gene marker. SOLUTION: The method includes the following process: primers are used, among 5'-TCT AGT GTT TGA GCA GAT AGG AAG GG-3' (Sequence No.1), an oligonucleotide composed of consecutive at least 10 bases, and among 5'-CTC TTA GTG GCA CTC CAT CAC ACT G-3' (Sequence No.2), an oligonucleotide composed of consecutive at least 10 bases, a nucleic acid amplification reaction process with a flatfish-derived nucleic acid as template is conducted, and the resultant amplification product is analyzed. COPYRIGHT: (C)2010,JPO&INPIT |
