PURINE NUCLEOSIDE PHOSPHORYLASE AS ENZYMATIC ACTIVATOR OF NUCLEOSIDE PRODRUGS
摘要
A process for inhibiting a mammalian cancerous cell or virally infected cell includes providing a Trichomonas vaginalis purine nucleoside phosphorylase enzyme or a tail mutant purine nucleoside phosphorylase enzyme in proximity to the mammalian cancerous cell or the virally infected cell and exposing the enzyme to a purine nucleoside phosphorylase enzyme cleavable substrate to yield a cytotoxic purine analog. The process includes introducing to the cell a vector containing the phosphorylase enzyme, or a DNA sequence coding for the same and delivering to the cell an effective amount of the substrate such as 9-(ß-D-arabinofuranosyl)-2- fluoroadenine (F-araA).
申请公布号
WO2010019954(A3)
申请公布日期
2010.06.10
申请号
WO2009US54058
申请日期
2009.08.17
申请人
THE UAB RESEARCH FOUNDATION;SOUTHERN RESEARCH INSTITUTE;PARKER, WILLIAM, B.;SORSCHER, ERIC, J.