ETHANOL RESISTANCE SACCHAROMYCES CEREVISIAE GP-01 BY PROTOPLAST FUSION, METHOD FOR MANUFACTURING THEREOF, METHOD FOR MANUFACTURING YEAST CONTAINING HIGH CONTENT OF BIO ORGANIC GERMANIUM BY USING SACCHAROMYCES CEREVISIAE GP-01 AND HIGH WATER SOLUBLE S

摘要

The present invention relates to a method for producing yeast containing the high content of organic bio-germanium by using yeast with ethanol-resistant and Sodium metagermanate(Na2 GeO3) by protoplast fusion. In order to enhance the resistance against ethanol which inhibits the growth of yeast, the present invention provides a method for producing a mutated Sac- charomyces Cervisiae(ye,d&t GP-Ol, KCTC 11399BP) which is ethanol-resistant and obtained by protoplast fusing Saccharomyces Cerevisiae(KCTC 7904) and Candida Ethanolica(KCTC 7181). Further, the present invention provides yeast containing the high content of organic bio- germanium(the yeast Ge-32K) and a method for producing the yeast Ge-32K, comprising steps of adding the yeast GP-Ol into a solution of sodium metagermanate(Na2Ge03) at the volume ratio of 1:0.5-2; adding 0.1~0.4wt% of surfactant, instead of germanium dioxide as used in the prior arts; and cultivating an obtained broth. The obtained yeast Ge-32K contains the higher content of the organic bio-germanium than the conventional yeast as produced by using germanium dioxide.