SINGLE MOLECULE PROTEIN SCREENING

摘要

Methods for improving single molecule protein analysis are disclosed. These methods can be used for discovery of new biomarkers, quantitation, and high throughput screening. By way of this invention, surface bound peptides are able to be directly sequenced using a modified Edman degradation followed by detection, e.g., labeled antibody detection. High throughput screening is enabled using pools of molecules (e.g., labeled antibodies) to identify and quantitate individual protein analytes in a biological sample.