摘要 |
The object is to increase the amount of a protein produced by recombinant DNA method using an eukaryotic cell, particularly a mammalian cell. The object is achieved by using a moderately low temperature transcription control element comprising the nucleotide sequence: X1cccX5X6x7 (wherein x1 represents c t or a; x5 represents g, c, a, or t; x6 represents c, t, a or g; and x7 represents, c, a, g, or t) or a nucleotide sequence complementary to the nucleotide sequence or moderately low temperature transcription enhancer having the moderately low temperature transcription control element linked thereto.
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