发明名称 |
METHODS FOR DIGITAL PCR |
摘要 |
The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a "droplet-in-oil" technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point. |
申请公布号 |
WO2010018465(A3) |
申请公布日期 |
2010.06.10 |
申请号 |
WO2009IB07568 |
申请日期 |
2009.08.11 |
申请人 |
STOKES BIO LIMITED;DAVIES, MARK;DALTON, TARA |
发明人 |
DAVIES, MARK;DALTON, TARA |
分类号 |
C12Q1/68;B01L3/00;G01N35/08 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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