发明名称 |
Zein-based peptide tags for the expression and purification of bioactive peptides |
摘要 |
Zein-based peptide tags, referred to here as inclusion body tags (IBTs), are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
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申请公布号 |
US7732569(B2) |
申请公布日期 |
2010.06.08 |
申请号 |
US20060641936 |
申请日期 |
2006.12.19 |
申请人 |
E.I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
DECAROLIS LINDA JANE;FAHNESTOCK STEPHEN R.;WANG HONG |
分类号 |
C07K1/00 |
主分类号 |
C07K1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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