SKIN IRRITATION FACTOR WITH YELLOW SANDS, AND THE METHODS FOR INDUCING THE SKIN IRRITATION AND SCREENING THE MATERIAL OF INHIBITING THE SKIN IRRITATION FACTOR, ALLEVIATING THE SKIN IRRITATION IN THE KERATINOCYTE, AND THE COMPOSITION FOR SKIN EXTERNAL APPLICATION CONTAINING THE MATERIAL SCREENED BY THE METHOD

摘要

PURPOSE: A method for screening a material which induces skin irritation by yellow dust is provided to reduce skin irritative factor expression and to reduce skin irritation by yellow dust. CONSTITUTION: A method for screening a material which induces skin irritation by yellow dust comprises: a step of treating yellow dust to keratinocytes; and a step of screening a gene of which expression level is significantly changed comparing with the expression of normal keratinocytes through RT-PCR. The material which induces skin irritation by yellow dust is AhR or CYP. A method for screening a material which relieves skin irritation by yellow dust comprises: a step of treating yellow dust to test cells to induce overexpression of an irritative factor; a step of treating a candidate material to the overexpressed cells; a step of measuring expression level of the irritative factor by RT-PCR. The material which relieves skin irritation by yellow dust is Trichosanthis semen. An external use skin composition for relieving skin irritation contains 0.001-1 weight% of Trichosanthis semen.