DEVICE FOR DETERMINING A FLUORESCENCE POLARIZATION ANISOTROPY DISTRIBUTION IN REAL TIME AND RELATED PROCEDURE FOR MEASURING IN REAL TIME A TEMPERATURE DISTRIBUTION OF A FLUID MEDIUM

摘要

The present invention refers to a device for determining a fluorescence polarization anisotropy distribution in real time of a medium of interest (104). Said device comprises: a light producing means (102) to induce a fluorescence emission of the medium of interest (104), a sensing means (106) to measure the fluorescence intensity, an optical imaging means (105) which conjugates optically at least part of the medium of interest (104) with a photosensitive part of the sensing means (106), and an active polarizing means (103) which modulates periodically the fluorescence intensity measured by the sensing means (106). Additionally, the invention refers to a procedure for measuring in real time a temperature distribution of a fluid medium of interest (104). Said procedure comprises the steps of : incorporating fluorescent temperature nanoprobes in the medium of interest (104), inducing the fluorescent temperature nanoprobes to emit fluorescence, measuring the fluorescence using an optical imaging means (105) and an active polarizing means (103) modulating said active polarizing means (103) periodically, extracting a fluorescence polarization anisotropy from the modulated fluorescence intensity.