| 摘要 |
The present invention provides novel compositions and methods that enable rapid production of proteins and RNA. In particular, a novel system is provided comprised of Cystovir?s procapsids alone or with p8 and single-stranded (ss) RNA (ssRNA) encoding a gene of interest. The ssRNA is uploaded by the procapsids and converted to double-stranded (ds) RNA (dsRNA), thereby producing functional capsids. The resulting capsids are useful for expression of recombinant proteins, such as enzymes, hormones, cytokines, adjuvants and vaccine antigens, and RNA, such as catalytic RNA, antisense RNA, siRNA, hairpin loops and microRNA, in cell-free systems as well as in plant, insect and mammalian cells. |
