AQUEOUS TWO PHASE EXTRACTION AUGMENTED PRECIPITATION PROCESS FOR PURIFICATION OF THERAPEUTIC PROTEINS
摘要
<p>The invention relates to an aqueous two phase extraction (ATPE) augmented precipitation process, which may be used to recover and also partially purify therapeutic proteins, including monoclonal antibodies from a crude multi-component mixture. The process involves the formation of a forward extraction PEG-Phosphate ATPE system in which the target product is preferentially partitioned to the polymer rich phase. A second ATPE back extraction system is then formed by introducing the polymer rich phase from the forward extraction to a new phosphate salt rich phase, causing the product to precipitate at the interface between the two phases. This precipitate is then recovered and resolubilised in a suitable buffer and may be passed on for further purification.</p>
申请公布号
WO2010062244(A1)
申请公布日期
2010.06.03
申请号
WO2009SE51305
申请日期
2009.11.18
申请人
GE HEALTHCARE BIO-SCIENCES AB;TRAN, RICHARD;TITCHENER-HOOKER, NIGEL, J.;LACKI, KAROL
发明人
TRAN, RICHARD;TITCHENER-HOOKER, NIGEL, J.;LACKI, KAROL