SELECTION OF TARGET GENE FOR ANTI-FUNGAL AGENT, AND METHOD FOR PREDICTING FUNCTION OF THE TARGET GENE

摘要

A method comprising the following steps 1) to 6): 1) selecting a candidate gene for a target gene; 2) selecting a gene that is induced specifically by the administration of a reference enzyme-type inhibitor as a candidate gene for a reporter gene; 3) constructing a vector having promoters for the candidate genes and a marker gene introduced therein; 4) introducing the vector to produce a transformant; 5) introducing deletion disruption or conditional disruption into the transformant; and 6) selecting a target gene for an anti-fungal agent based on the difference in growth between the transformant and a cell that is of the same type as the transformant and does not have deletion disruption or conditional disruption introduced therein or, alternatively, 6) predicting the function of a target gene for an anti-fungal agent based on the difference in the expression level of the marker gene between the transformant and a cell that is of the same type as the transformant and does not have deletion disruption or conditional disruption introduced therein.