COMPOSITION AND METHOD FOR ENHANCED SECRETION OF PEPTIDES AND PROTEINS FROM BACTERIA

摘要

This invention provides compositions and methods for achieving enhanced delivery of desired protein or peptide products from an expression system delivered by means of an appropriate bacterial host where a novel leader peptide comprising the sequence MGKKNLRIVSAAAAALLAVAPVAATAMPVNAA (SEQ ID: 1) is operatively linked to the peptide or protein sequence to achieve enhanced expression and secretion of the product from the bacterium. Included in the system according to this invention is a bacterium which auto-induces expression of a genetic construct encoding the leader peptide in operative association with a peptide or protein product the expression and secretion of which is desired from the bacterium.