METHOD OF DETECTING METHYLATED CYTOSINE

摘要

The method for detecting a methylated cytosine of the present invention comprises the steps of:
hybridizing a sample DNA with an oligonucleotide which can hybridize with a region of the sample DNA containing a cytosine suspected of being methylated and has an abasic site at the position complementary to the cytosine;
reacting the hybridized sample DNA obtained in the previous step with an oxidizing agent to oxidize the cytosine when it is methylated; and
detecting the oxidized methylated cytosine.