发明名称 |
Nested oligonucleotides containing a hairpin for nucleic acid amplification |
摘要 |
Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library of polypeptides or proteins, such as, for example, an antibody library.
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申请公布号 |
USRE41365(E1) |
申请公布日期 |
2010.06.01 |
申请号 |
US20090378811 |
申请日期 |
2009.02.18 |
申请人 |
ALEXION PHARMACEUTICALS, INC. |
发明人 |
BOWDISH KATHERINE S.;FREDERICKSON SHANA;MCWHIRTER JOHN;MARUYAMA TOSHIAKI;LIN YING-CHI |
分类号 |
C12P19/34;C07H21/04;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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