发明名称 |
MANIPULATION OF SNF1 PROTEIN KINASE ACTIVITY FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS |
摘要 |
<p>Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [PUFAs], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 ?-subunit, Gal83 ß-subunit or Snf4 ?-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.</p> |
申请公布号 |
WO2010025374(A3) |
申请公布日期 |
2010.05.27 |
申请号 |
WO2009US55376 |
申请日期 |
2009.08.28 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY;HONG, SEUNG-PYO;SEIP, JOHN, E.;ZHU, QUN |
发明人 |
HONG, SEUNG-PYO;SEIP, JOHN, E.;ZHU, QUN |
分类号 |
C12P7/64;C12N15/09;C12N15/81 |
主分类号 |
C12P7/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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