METHOD OF DETERMINING PEGYLATED BLOOD COAGULATION FACTOR ACTIVITY IN A SILICA-BASED ACTIVATED PARTIAL THROMBOPLASTIN TIME ASSAY

摘要

Provided is a method of measuring coagulation activity of a PEGylated blood coagulation factor through a silica based activated partial thromboplastin time (APTT) assay. A PEGylated blood coagulation factor was found to display reduced activity in a silica-based APTT assay, and the addition of PEG to the APTT reaction mixture was shown to restore its activity. Methods for determining the coagulation activity of a PEGylated blood coagulation factor in an APTT assay are provided.