RECOMBINANT MOUSE INTERLEUKIN-6 IMPROVE VIABILITY IN MOUSE FERTILIZED AND CLONED EMBRYOS

摘要

PURPOSE: An in vitro culture system of mouse fertilized egg and cloned egg is provided to improve in vitro culture system and to enhance survival rate of the cells by adding a recombinant interleukin-6 to a basic medium(M16). CONSTITUTION: A mouse fertilized egg is cultured by adding recombinant mouse interleukin-6 to a basic medium(M16) and culturing initial fertilized egg and cloned egg in vitro without BSA(bovine serum albumin). The medium of the fertilized egg is M2 medium for washing and M16 medium for culturing.