METHOD FOR ISOLATING THE DNA OF THE AVIAN INFECTIOUS LARYNGOTRACHEITIS VIRUS FOR PCR ANALYSIS

摘要

The invention relates to molecular biology, more particularly to DNA isolation methods. The method involves isolating the DNA of the avian ILT virus from virus-containing materials by lysing and inactivating proteases using a lysing buffer which consists of guanidine isothiocyanate, and subsequently absorbing the DNA on silicon dioxide particles. The proposed method is more effective than known procedures for isolating viral DNA for PCR and can be used in laboratories and science and research institutions dealing with PCR diagnostics of DNA-containing viral infections. Said DNA isolation method is easy to use, cost-effective and workable and can be used for preparing samples for amplification.