| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for screening a compound interacting with a transmembrane protein, and for determining whether a protein such as the transmembrane protein can form an oligomer or not. <P>SOLUTION: In the method for screening a candidate compound interacting with at least one kind of transmembrane protein, first of all, a transmembrane protein containing at least one nuclear localization sequence (NLS) and a detectable moiety is expressed in a cell, and then, a candidate compound is brought into contact with the cell, and further, a distribution of the detectable moiety in the cell is detected, to thereby determine a distribution of an expressed protein in the cell. Concerning determination of whether a first protein and a second protein can form an oligomer or not, it can be determined whether the oligomer can be formed or not, by detecting the detectable moiety in a nucleus or near the nucleus of the cell, or by knowing occurrence of an interaction between the first protein and the second protein by detecting decline of a level of the detectable moiety on the cell surface, relative to a control cell. <P>COPYRIGHT: (C)2010,JPO&INPIT</p> |
