DEVICE FOR MEASUREMENT OF FERTILE EGG RESPIRATORY ACTIVITY AND METHOD FOR MEASUREMENT OF FERTILE EGG RESPIRATORY ACTIVITY

摘要

Disclosed are a device for measurement of the respiratory activity of a fertile egg and a method for measurement of the respiratory activity of a fertile egg, which are able to accurately measure the amount of respiration of a fertile egg while taking into account the oxygen reduction current of an electrode, for which the appropriateness of the measurement results can be verified, and which provides an improvement with respect to non-invasiveness. A chip (11) for measurement of the respiration of fertile eggs is formed with electrodes (13) disposed on a substrate (12) and has in the vicinity of the electrodes (13) a PDMS well (14) or a PDMS micro flow channel (15) for the purpose of introducing embryos. The substrate (12) is comprised of a quartz glass substrate the surface of which is covered by means of an SiO2 deposition insulation film such that the electrodes (13) are exposed. The upper limit value for the oxygen reduction current value of the electrodes (13) is set, based on the amount of oxygen consumed in response to the oxygen reduction reaction thereof and the amount of oxygen consumed by the embryos, such that the influence of the amount of oxygen consumed in response to the oxygen reduction reaction on the amount of oxygen consumed by the embryos can be ignored.