| 摘要 |
The present invention is related to a method for quantifying the amount of at least one target protein in a sample comprising the steps of:
1. digesting the sample to provide peptides,
2. preparing at least one labeled monitor peptide comprising a subsequence of said target protein(s) to provide an internal standard,
3. adding an aliquot of the product of step 2 containing a known amount of labeled synthetic peptide to the product of step 1,
4. loading the product of step 3 onto at least one support which has attached thereto at least one binding agent which binds to at least one monitor peptide,
5. washing the support(s) of step 4 to remove unbound peptides,
6. eluting the monitor peptide(s) from the binding agent(s) bound to the support(s),
7. subjecting at least one of the monitor peptides present in the eluate(s) obtained in step 6 to mass spectrometry to determine the amount of monitor peptide(s) in the digest by comparison with the labeled synthetic standard(s). |
