α-isomaltosylglucosaccharide-forming enzyme, process and uses of the same

摘要

The object of the present invention is to provide anα-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing anα-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both theα-1,6 glucosidic linkage as a linkage at the non-reducing end and theα-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing theα-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having theα-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power;α-isomaltosyl-transferring method using the enzyme; method for formingα-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both theα-isomaltosylglucosaccharide-forming enzyme and theα-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.