Provided herein are methods for cleaving a target RNA polynucleotide. The target RNA polynucleotide includes a Cas6 recognition domain and a cleavage site, and may be based on a repeat from a CRISPR locus. The methods may be practiced in vivo or in vitro. Also provided are polypeptides that have Cas6 endoribonuclease activity in the presence of a target RNA polynucleotide, and methods for using the polypeptides.
申请公布号
WO2010054108(A2)
申请公布日期
2010.05.14
申请号
WO2009US63432
申请日期
2009.11.05
申请人
UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.;TERNS, REBECCA, M.;TERNS, MICHAEL, P.;CARTE, JASON
发明人
TERNS, REBECCA, M.;TERNS, MICHAEL, P.;CARTE, JASON