Various embodiments of the present teachings relate to methods for the methylation analysis of nucleic acids. The subject methods include methods that result in the preparation of mate-pair libraries suitable for highly multiplexed DNA sequencing. Embodiments include methods of preparing mate-pair libraries comprising a first tag sequence and a second tag sequence, wherein one of the tag sequences has been converted by a methylation conversion agent and the other tag sequence has not been converted by the methylation conversion agent. Other embodiments provided include intermediates for making the mate-pair library and kits for making the mate-pair libraries. Also provided is software and computer systems for analyzing the methylation levels of genomic DNA from which the tag sequences were derived.
申请公布号
WO2010003153(A3)
申请公布日期
2010.05.14
申请号
WO2009US49724
申请日期
2009.07.06
申请人
LIFE TECHNOLOGIES CORPORATION;MCKERNAN, KEVIN, J.;SCHROEDER, BENJAMIN, G.;BOYD, VICTORIA, L.
发明人
MCKERNAN, KEVIN, J.;SCHROEDER, BENJAMIN, G.;BOYD, VICTORIA, L.