Method and System for Internal Standardization of Assays

摘要

Internal standard concentration is normalized as an independent parameter through measuring a buffer conductance in a system with characterized reagents and in a defined assay device. The conductivity of a fresh internal standard solution is measured whether in isolation or as part of a system that is subject to a uniform variation such as evaporation or condensation. Collected conductance measurements may be used to normalize assay signals that are dependent upon fluidic channel dimensions, such as fluorescence assay signal measurement.