| 摘要 |
A microscope for conventional fluorescence microscopy (epi-fluorescence) and for total internal reflection microscopy, comprising at least one light source (1) for the conventional fluorescent illumination and at least one light source (2) for the evanescent illumination, and comprising an objective (4), wherein the illumination light coming from the light sources (1, 2) on different illumination paths (5, 6) is passed via a beam combiner (7) into the objective (4) and from there to the specimen (8), the beam combiner (7) being structured such that it directs the illumination light used for the conventional fluorescent illumination and the illumination light used for the evanescent illumination into the objective (4) in geometrically separated beam paths (5, 6), is characterized in that the beam combiner (7) has at least two spatially separated zones (9, 10) for coupling in the illumination light used for the conventional fluorescent illumination and at least two spatially separated zones (11, 12) for coupling in the illumination light used for the evanescent illumination, the individual zones being adapted in their size and position to the objective pupils of different objectives (4).
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