| 摘要 |
The Candida and Aspergillus polypeptides of the invention have been found to be immunogenic and are useful as diagnostic test antigens. The polypeptide antigens of the subject invention can provide the basis of a diagnostic assay that would allow the rapid, in-house, laboratory diagnosis of infection with Candida and/or Aspergillus using a sample (e.g., serum, plasma, or whole blood) from an infected human or animal. Additionally, the subject invention provides methods of detecting the presence of Candida albicans and/or Aspergillus fumigatus in biological or environmental samples utilizing antibodies provided by the subject invention. Furthermore, the use of single antigens or, more preferably, one or more groups (sets) of antigens of the invention in the diagnosis of these important diseases offers many advantages including enhanced test specificity, ease of testing and consistency of results using synthetically or recombinantly produced test antigens instead of cultured, whole organisms. In one embodiment, the group of antigens comprises or consists of one or more polypeptides (e.g., one, two, three, or four or more antigens) selected from among SET1 (chromatin regulatory protein), ENO1 (enolase I), PGK1-2 (phosphoglycerate kinease), and MUC1-2 (cell surface glycoprotein). In another embodiment, the group of antigens comprises or consists of one or more polypeptides (e.g., one, two, three, four, five, six, or seven or more polypeptides) selected from among SET1, ENO1, FBA1, PGK1-1, PGK1-2, MUC1-2, and BGL2. In another embodiment, the group of antigens comprises or consists of one or more polypeptides (e.g., one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, or fifteen or more polypeptides) selected from among MET6-1, MET6-2, NOT5, RBT4, IPF9162, CAR1, GAP1, SET1, ENO1, FBA1, PGK1-1, PGK1-2, MUC1-1, MUC1-2, and BGL2.
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