| 摘要 |
The present disclosure relates, according to some embodiments, to a method of stabilizing a molecule (e.g., a biomolecule) in a bodily fluid comprising: (1) providing a stabilizing solution comprising: (a) an amount of a divalent metal chelator selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), (ethylenebis(oxyethylenenitrilo))tetraacetic acid (EGTA), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and salts thereof in the range of from about 0.001 M to about 2 M; and (b) an amount of at least one chelator enhancing component selected from the group consisting of lithium chloride, guanidinium chloride, guanidinium thiocyanate, sodium salicylate, sodium perchlorate, and sodium thiocyanate in the range of from about 0.1 M to about 10 M; and (2) adding the stabilizing solution to the bodily fluid, thus stabilizing the molecule. A biomolecule may be selected from a nitrite, a carbohydrate, a ketone, a globin, a bilirubin, a lipid, and combinations thereof in some embodiments. |
