| 摘要 |
The invention concerns a method and an apparatus for the quantitative determination of the concentration of fluorophores of at least one substance in a sample. A constant portion of the reference light of a reference light wave length (λr) emitted by a reference light source (28) is coupled in by an optical element (REM) in the direction of a receiving element (38). A first measured value is detected which corresponds to the portion of the reference light coupled in which is incident on the receiving element (38). The sample is irradiated with the excitation light of an excitation wave length (λex) emitted by an excitation light source (26). A second measured valued is detected which corresponds to the portion of the fluorescent light of an emission wave length (λem) emitted by the sample which is incident on the receiving element (38). The ratio (Emes2/Es2) of the second measured value (Emes2) to the first measured value (Es2) is determined. The number of fluorophores in the substance of the sample within the detection zone is determined taking into account the determined ratio (Emes2/Es2).
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