| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for judging gene polymorph, capable of accurately judging the mating type of polymorph part without concerning to the DNA amount. <P>SOLUTION: The method for judging the mating type of the gene polymorph comprises performing invader reaction generating a first fluorescence derived from a first oligonucleotide and a second fluorescence derived from a second oligonucleotide by mixing the target DNA with the first oligonucleotide complementary to the target sequence containing a first allele composing the gene polymorph of the target DNA and the second oligonucleotide complementary to the target sequence containing a second allele forming the gene polymorph and controlling the temperature, approximating the curves representing temporal changes of the first and second fluorescence intensities at the predetermined judging section after the reaction start are approximated by quadratic equations, respectively, and using the ratio of the secondary coefficient of the first and second secondary approximation formulae as the judging index, or performing secondary differentiation of each of the curves representing temporal changes of the first and second fluorescence intensities and using the ratio of the maximum values of the first and second secondary differentiation values as the judging index. <P>COPYRIGHT: (C)2010,JPO&INPIT |
