| 摘要 |
In the process of identifying a protein by analyzing and processing mass spectrum data obtained for each micro area (pixel) created by subdividing a two-dimensional area on a sample, mass windows including a peak or peaks on the mass spectrum of each pixel are set (S10), and an integrated value of the ion intensities of the peaks included in each mass window is calculated (S11). For each mass window, a mapping image is created by collecting the integrated intensity values of the pixels (S12), and the mass windows are grouped by evaluating the similarity of the mapping images (S13 and S14). The peaks included in the mass windows belonging to the same group are regarded as originating from the same kind of substance, and those peaks are collected to create a mass spectrum (S15). Based on this spectrum, a protein is identified by a PMF method or the like. The present method can achieve a high level of identifying accuracy even if two or more kinds of proteins are mixed together.
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