MULTIPLEX GENOTYPING ASSAY FOR DETECTING MUTATIONS IN K-RAS

摘要

A novel multiplex genotyping assay for the detection of mutations in the K-ras gene is disclosed. The assay may be used to predict the responsiveness of cancer patients to various treatments such as therapy targeting the epidermal growth factor receptor (EGFR). The assay may be performed in a single tube PCR reaction and has a number of advantages including low cost, high throughput and speed, reduced complexity, ease of interpretation of results and amenability to challenging specimen templates such as formalin-fixed paraffin-embedded (FFPE) tissue.