| 摘要 |
A method for determining a centroid coordinate of an image spot, comprising a) determining a search region having a border, b) identifying a first pixel in the search region, the pixel having a first intensity value, c) determining an upper threshold intensity value greater than the first intensity value, d) searching for a second pixel within the search region having an intensity value that is greater than the upper threshold intensity value, and e) upon finding the second pixel, designating the first pixel an integration region center.
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