| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of determining the base sequence of DNA using a cycle sequence reaction, which method exhibits a high signal strength. SOLUTION: The method of determining the base sequence of DNA using a cycle sequence reaction includes a process of subjecting a solution comprising DNA wherein the base sequence is to be determined to a heat treatment, a process of subjecting the same to a cooling treatment, and a process of carrying out a cycle sequence reaction by adding heat-resistant DNA polymerase, deoxyribonucleotide triphosphates with respect to each of the four species of the bases, and dideoxyribonucleotide triphosphates with respect to each of the four species of the bases thereinto. COPYRIGHT: (C)2010,JPO&INPIT |
