METHOD OF GENERATING AND SCREENING FOR PROTEASE WITH ALTERED SPECIFICITY

摘要

PROBLEM TO BE SOLVED: To provide methods of generating and screening proteases, which are enzymes that cleave substrate sequences. SOLUTION: The method of identifying protease includes: the step for producing a library of proteases, wherein each member of the library has a protease skeleton having mutations of N to the wild type skeleton sequence; the step for measuring activity of each member of the library when cleaving the substrate sequence; and the step for comparing the activity of each member to the average activity of the library and for identifying which protease has highest cleaving activity. COPYRIGHT: (C)2010,JPO&INPIT