Real-time high multiplex detection by primer extension on solid surfaces

摘要

The present invention provides a method that allows for real-time detection of a multitude of target nucleic acids of interest in one reaction (multiplexing) using detectable labels of a single color. The method comprises directly amplifying one or more target nucleic acids on a solid substrate thus providing an enhanced signal to noise ratio and reduced assay times. Further provided is a diagnostic kit for monitoring the amplification of one or more target nucleic acids.