发明名称 |
NUCLEASE REDUCTION |
摘要 |
A method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth comprising a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol; c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C.; whereby the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 80% of the initial value. |
申请公布号 |
EP2181191(A1) |
申请公布日期 |
2010.05.05 |
申请号 |
EP20080787332 |
申请日期 |
2008.08.19 |
申请人 |
NOVOZYMES A/S |
发明人 |
HANSEN, KIM, UHRE;OESTERGAARD, PETER, RAHBEK |
分类号 |
C12N9/54;C12N9/22;C12N9/56;C12N9/96 |
主分类号 |
C12N9/54 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|