发明名称 |
Process for producing recombinant protein in bacterium belonging to the genus Rhodococcus |
摘要 |
Provided is an expression vector capable of constitutive expression of a foreign gene in a bacterium belonging to the genus Rhodococcus. The vector is a constitutive expression vector for a bacterium belonging to the genus Rhodococcus comprising: DNA comprising a nucleotide sequence of a mutated TipA gene promoter where a mutation is incorporated into a −10 region sequence of a TipA gene promoter, the mutated TipA gene promoter being capable of thiostrepton-independent and constitutive expression of a gene located downstream thereof; a promoter sequence for the constitutive expression of a foreign gene; a ribosome-binding site sequence located downstream of the promoter sequence; and a multiple-cloning site sequence capable of incorporating a foreign gene therein, located downstream of the ribosome-binding site sequence.
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申请公布号 |
US7709624(B2) |
申请公布日期 |
2010.05.04 |
申请号 |
US20050553979 |
申请日期 |
2005.10.20 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY |
发明人 |
NAKASHIMA NOBUTAKA;TAMURA TOMOHIRO |
分类号 |
C07H21/04;C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/63;C12N15/74;C12P21/02;C12R1/01 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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