Methods for the production of ansamitocins

摘要

Provided is a process for preparing purified ansamitocins of a specified structure, comprising the steps of: 1) culturing an ansamitocin-producing microorganism in a liquid culture medium; 2) solid phase extraction of ansamitocins from the culture medium onto a hydrophobic resin; 3) isocratic or gradient elution of ansamitocins from the resin using an organic solvent or an organic solvent combined with water; 4) concentrating the extracted ansamitocins; and 5) optionally purifying the ansamitocins by anyone of a) b), c) and d): a) adsorption chromatography over silica gel or alumina, b) crystallization, c) adsorption chromatography over silica gel or alumina followed by crystallization, and d) crystallization followed by adsorption chromatography over silica gel or alumina. Further provided are variants of the method and specific temperatures and solvents for the purification process.