| 摘要 |
PURPOSE: A live vaccine composition containing bacteria incapable of surviving at the environment is provided to easily produce and store the vaccine, and to safely and effectively prevent diseases when injecting to domestic animals. CONSTITUTION: A live vaccine composition containing auxotrophic bacteria which is lack of essential genes necessary for the outgrowth of a cell, and need to be supplied with the essential genes from a auxotrophy recombinant vector. The auxotrophic bacteria are either the Salmonella typhi, the S. typhimurium, the S. enteritidis, the S. galinarum, the S. dublin, the S. Cholerae-suis or the S. pullorum. The essential genes necessary for the outgrowth of the cell is aspartate kinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate reductase, glutamate:succinyl-diaminopimelate aminotransferase, or succinyldiaminopimelate desuccinylase.
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