Method of testing cardiomyocytes derived from human embryonic stem cells formed in embryoid bodies differentiated into all of the major cardiac muscle cell types, including ventricular, atrial, and nodal cells

摘要

Disclosed is a method for testing an agent for its effect on human cardiac cells comprising the steps of culturing aggregates of approximately 500-800 undifferentiated human pluripotent embryonic stem cell to produce embryoid bodies. Differentiating the embryoid bodies in vitro for 40-95 days to derive atrial, ventricular and nodal cardiomyocte cell types. A single cardiomyocyte is then measured for its transmembrane potential using an electrode then exposed to an agent and observing whether the action potential of the cardiomyocyte changes.