METHODS FOR PREVENTING AGGREGATION OF ADIPOSE STROMAL CELLS

摘要

The present invention relates to a methods, compositions, and admixtures which prevent the aggregation of a population of adipose stromal cells (ASCs), such as an isolated population of adipose stromal cells. In some embodiments, the present invention relates to admixtures and methods of use thereof comprising a population of ASC and a modulator of ASC aggregation. In some embodiments, a modulator of ASC aggregation includes, for example, ionic agents (e.g., heparin), chelating agents (e.g., EDTA), proteolytic agents. (e.g., trypsin or dispase), and agents which inhibit the expression of cell surface receptors and molecules on the surface of ASCs (e.g., inhibitors of integrins expression). In some embodiments, the methods to block ASC aggregration and the admixtures are useful in use of the ASCs to treat various diseases and/or conditions, and increases the safety and/or efficiency of ASCs. The methods, compositions and admixtures have utility in treating a number of conditions including, but not limited to, the treatment of any vascularized organs, such as heart, kidney, liver and the like.